![]() Negative control: Absence of encapsulated yeastsĬapsules of Cryptococcus neoformans like organisms seen Importance of India Ink Preparation Assay.Positive control: Presence of encapsulated yeasts.The encapsulated strain of Cryptococcus neoformans in India Ink preparation of CSF Result Interpretation of India Ink Preparation The encapsulated strain of Cryptococcus neoformans in India Ink preparation of CSF Fig. Look for oval or round cells, some showing budding, irregular in size, measuring 2-10 µm in diameter, and surrounded by a large unstained capsule as shown in the figure. If any suspicious objects encounter, examine with the high dry objective (40X).Examine the entire 22- by 22-mm coverslip systematically with the low power objective (10X ) and low light intensity.a drop of the sediment to a slide and add a drop of India ink. Transfer an equal amount of sediment and India ink i.e.Remove the supernatant fluid and mix the sediment.Centrifuge the CSF for 5 to 10 minutes. ![]()
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